Discovery of electrophoresis


Electrophoresis is a comprehensive term that refers to the migration of charged solutes or particles of any size in a liquid medium under the influence of an electrical field.

The first electrophoresis method used to study proteins was the free solution or moving boundary method devised by Tiselius in 1937. The Tiselius apparatus was a rectangular) sectional U-tube equipped with sensitive Schlieren optics. It allowed changes in refractive index to be observed as segments of solution with different protein content migrated past. This technique was used in research to measure electrophoretic mobility and to study protein-protein interaction.

2 comments:

  1. Brown AmyFebruary 5, 2016 at 1:14 AM

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  2. UnknownFebruary 6, 2016 at 2:30 AM

    Electrophoresis is an analytical technique mainly employed in medicine and molecular biology field to isolate and characterize proteins, nucleic acids and other particles. By applying an electric field, the samples charged particles get migrate. The movement of charged particles has been formed on various medium like paper, gel or liquid. This process is very valuable in indentifying diseases in which the serum and plasma proteins are changed.

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